📊 Number of groups and replicates per group:  

(e.g.: Control, T1, T2 / Total: 5 groups×۳ replicates)

۱. Sample Preparation (denaturation in Laemmli buffer)
۲. Precast or Custom Gels (8%, 10%, 12%, or 15% acrylamide)  
۳. Precision Electrophoresis (constant voltage/current)
۴. Professional Staining (Coomassie Blue R-250 or Silver Stain)  
۵. High-Resolution Documentation (Gel scanner with UV/white light)  
۶. Molecular Weight Analysis (Precision ladder included)  

۱. Silver Staining (Higher sensitivity)  
۲. Urgent Processing (24-48h)  
۳. Gel Slicing (For mass spec or WB transfer)  
۴. Densitometry Report (Band intensity analysis)