✅ Western Blot Standard Package Note: (1+ Target antibodies) Up to 10 samples (e.g., 2 groups × 5 replicates)
1. Sample Preparation ( Lysis, protein quantification, and denaturation)
2. SDS-PAGE Electrophoresis – Customizable gel percentage (8%, 10%, 12%, or 15%)
3. Protein Transfer – PVDF or nitrocellulose membrane (your choice)
4. Antibody Incubation – Includes 1 primary antibody (customer-provided or ours)
– Includes 1 reference antibody (e.g., GAPDH/β-Actin)
– Blocking and secondary antibody (HRP/fluorescence)
5. Detection & Imaging
6.
Quantitative densitometry analysis (e.g., vs. β-actin/GAPDH)
7. Basic Analysis Band intensity measurement (raw data)